Wastewater - 40 CFR part 136

Inorganic Parameters

Tests Method Container Type Hold Time Preservation Locations Available
Acidity, Total 2310B P.G 14 days Cool, <=6C TN, LA, MS
Alkalinity, Total 2320B P,G 14 days Cool, <=6C TN, LA, MS
Bicarbonate 2320B P,G 14 days Cool, <=6C TN, LA, MS
Carbonate 2320B P,G 14 days Cool, <=6C TN, LA, MS
Total Bicarbonate/Carbonate 2320B P,G 14 days Cool, <=6C TN, LA, MS
Ash % (Ignition at 550C) 160.4 P,G 7 days Cool, <=6C TN
Biochemical Oxygen Demand (BOD) 5210B P,G 48 hours Cool, <=6C TN, LA, MS
Biochemical Oxygen Demand Carbonaceous (CBOD) 5210B P,G 48 hours Cool, <=6C TN, LA, MS
Biochemical Oxygen Demand Ultimate Inquire TN, LA
Bromide 300.0 P,G 28 days None Required TN, LA
Chemical Oxygen Demand (COD) 410.4 / 5220D P,G 28 days Cool, <=6C / H2SO4 pH<2 TN, LA, MS
Chloride 4500-Cl B P,G 28 days None Required TN, LA
Chloride 300.0 P,G 28 days None Required TN, LA
Chlorine, Total Residual 4500-Cl G P,G Field Cool, <=6C TN, LA, MS
Chlorine, Free 4500-Cl G P,G Field Cool, <=6C TN, LA, MS
Chromium, Hexavalent (Colormetric) 3500-Cr B P,G 28 days Cool, <=6C / NaOH pH of 9 TN, LA, MS
Color 2120B P,G 48 hours Cool, <=6C TN, LA, MS
Color (ADMI) 2120F P,G 48 hours Cool, <=6C TN, MS
Conductance (Specific) 2510B P,G 28 days Cool, <=6C TN, LA
Conductance (Resistivity) 2510B P,G 28 days Cool, <=6C TN, LA
Cyanide, Total 4500CNE P,G 14 days Cool, <=6C / NaOH pH>12 TN
Cyanide, Amenable 4500CNG P,G 14 days Cool, <=6C / NaOH pH>12 TN
Dissolved Oxygen 4500OC G Bottle and top Field NA TN, LA, MS
Fluoride (without Distillation) 4500FB P,G 28 days Cool, <=6C TN
Fluoride 300.0 P,G 28 days None Required TN, LA
Hardness by Calculation (Ca, Mg) 200.7 / 2340B P,G 6 months HNO3 to pH<2 TN
Hardness by Calculation (Ca, Mg) SM3111B/2340B P,G 6 months HNO3 to pH<2 TN, LA
MBAS (Surfactants) 5540C P,G 48 hours Cool, <=6C TN, MS
Nitrogen - Ammonia (NH3, undistilled, ISE) 4500 NH3D P,G 28 days Cool, <=6C / H2SO4 pH<2 TN, LA, MS
Nitrogen - Ammonia (NH3, distilled, titration) 4500 NH3C P,G 28 days Cool, <=6C / H2SO4 pH<2 TN
Nitrogen - Kjeldahl Total 4500 NH3D P,G 28 days Cool, <=6C / H2SO4 pH<2 TN
Nitrogen - Kjeldahl Total 351.1 P,G 28 days Cool, <=6C / H2SO4 pH<2 TN, MS
Nitrogen - Organic = TKN - NH3 Calculation P,G 28 days Cool, <=6C / H2SO4 pH<2 TN, MS
Nitrogen  Total = TKN + (NO2/NO3) Calculation P,G 28 days Cool, <=6C / H2SO4 pH<2 TN, MS
Nitrogen-Nitrate 300.0 P,G 48 hours Cool, <=6C TN, LA
Nitrogen-Nitrate 4500 NO3E P,G 28 days Cool, <=6C / H2SO4 pH<2 TN, LA
Nitrogen-Nitrite 300.0 P,G 48 hours Cool, <=6C TN, LA
Nitrogen-Nitrite 353.2 P,G 48 hours Cool, <=6C TN, MS
Nitrogen-Nitrate and Nitrite 353.2 P,G 48 hours Cool, <=6C TN, MS
Nitrogen-Nitrate and Nitrite 4500 NO3E P,G 48 hours Cool, <=6C TN, LA
Odor 140.1 G 24 hours Cool, <=6C TN, MS
Oil & Grease - HEM (Water) 1664A G 28 days Cool, <= 6C HCl pH<2 TN, LA, MS
Oil & Grease - SGT-HEM (TPH - Water) 1664A-SGT G 28 days Cool, <= 6C HCl pH<2 TN, LA, MS
Phenol - Total Colormetric 420.1 G 28 days Cool, <=6C / H2SO4 pH<2 TN
Phenol - Total Colormetric (Direct) 420.1 G 28 days Cool, <=6C / H2SO4 pH<2 TN
Ortho Phosphate 4500 PE P,G 28 days Cool, <=6C / H2SO4 pH<2 TN, LA, MS
Ortho Phosphate by IC 300.0 P,G 48 hours Cool, <=6C TN
Total Phosphorous 365.4 P,G 28 days Cool, <=6C / H2SO4 pH<2 TN, MS
Total Dissolved Solids (TDS) 2540C P,G 7 days Cool, <=6C TN, LA, MS
Total Suspended Solids (TSS) 2540D P,G 7 days Cool, <=6C TN, LA, MS
Total Solids (% Solids TS) 2540B P,G 7 days Cool, <=6C TN, LA, MS
Total Volatile Solids (TVS) 160.4 P,G 7 days Cool, <=6C TN, MS
Settleable Solids (SS) 2540E P,G 7 days Cool, <=6C TN, MS
Silica 200.7 P 28 days Cool, <=6C TN, MS
Specific Conductance 2510B P,G 28 days Cool, <=6C TN, LA
Specific Conductance 120.1 P,G 28 days Cool, <=6C TN, MS
Sulfate 300.0 P,G 28 days Cool, <=6C TN, LA
Sulfide 4500S2F P,G 7 days Cool, <=6C / zinc acetate + NaOH pH>9 TN
Sulfite 4500SO32B P,G Field None Required TN
Total Organic Carbon (TOC) - Water 5310C TN
Total Organic Carbon (TOC) - Water 5310D G 28 days Cool, <=6C / HCl pH<2 TN, LA
Total Organic Carbon (TOC) - Water 5310D G 28 days Cool, <=6C / HCl pH<2 TN, LA
Turbidity 2130B P,G 48 hours Cool, <=6C TN, LA
Turbidity 180.1 P,G 48 hours Cool, <=6C TN, MS

Organic Parameters

Tests Method Container Type Hold Time Preservation Locations Available
Volatile Organic Compounds (VOCs) 624 40ml Vial 14 days Cool, <=6C, HCl pH<2 TN
Semi-Volatile Organics (SVOCs/BNAs) 625 Amber Liter 7/40 days Cool, <=6C, Na2S2O3 TN
Acid (Phenolic) Fraction 625 Amber Liter 7/40 days Cool, <=6C, Na2S2O3 TN
Base/Neutral Fraction 625 Amber Liter 7/40 days Cool, <=6C, Na2S2O3 TN
Polynuclear/Aromatic Hydrocarbons (PAH) 625 Amber Liter 7/40 days Cool, <=6C, Na2S2O3 TN
2,3,7,8-TCDD (Screen Only) 625 Amber Liter 7/40 days Cool, <=6C, Na2S2O3 TN
Organochlorine Pesticides and PCBs 608 Amber Liter 7/40 days Cool, <=6C, Na2S2O3 TN
Organochlorine Pesticides 608 Amber Liter 7/40 days Cool, <=6C TN
PCBs 608 Amber Liter 7/40 days Cool, <=6C TN
Total Toxic Organics (TTO) 624/625/608 See above See Above TN
Total Toxic Organics (w/o Pest/PCBs) 624/625 See above See Above TN

Metals - ICP/FLAA/CV

Tests Method Container Type Hold Time Preservation Locations Available
Aluminum 200.7 Plastic 6 months HNO3 to pH<2 TN
Antimony 200.7 Plastic 6 months HNO3 to pH<2 TN
Arsenic 200.7 Plastic 6 months HNO3 to pH<2 TN
Barium 200.7 Plastic 6 months HNO3 to pH<2 TN
Beryllium 200.7 Plastic 6 months HNO3 to pH<2 TN
Boron 200.7 Plastic 6 months HNO3 to pH<2 TN
Cadmium 200.7 Plastic 6 months HNO3 to pH<2 TN
Calcium 200.7 Plastic 6 months HNO3 to pH<2 TN
Calcium SM 3111B Plastic 6 months HNO3 to pH<2 LA
Chromium Total 200.7 Plastic 6 months HNO3 to pH<2 TN
Cobalt 200.7 Plastic 6 months HNO3 to pH<2 TN
Copper 200.7 Plastic 6 months HNO3 to pH<2 TN
Iron 200.7 Plastic 6 months HNO3 to pH<2 TN
Lead 200.7 Plastic 6 months HNO3 to pH<2 TN
Magnesium 200.7 Plastic 6 months HNO3 to pH<2 TN
Magnesium SM 3111B Plastic 6 months HNO3 to pH<2 LA
Manganese 200.7 Plastic 6 months HNO3 to pH<2 TN
Mercury (Water) 245.1 Plastic 28 days HNO3 to pH<2 TN
Mercury, Low Level 1631E Amber 250 mL 90 Days 5 ml/L 12N HCl or 5 ml/L BrCl MS
Molybdenum 200.7 Plastic 6 months HNO3 to pH<2 TN
Nickel 200.7 Plastic 6 months HNO3 to pH<2 TN
Phosphorous 200.7 Plastic 6 months HNO3 to pH<2 TN
Potassium 200.7 Plastic 6 months HNO3 to pH<2 TN
Potassium SM 3111B Plastic 6 months HNO3 to pH<2 LA
Selenium 200.7 Plastic 6 months HNO3 to pH<2 TN
Silver 200.7 Plastic 6 months HNO3 to pH<2 TN
Sodium 200.7 Plastic 6 months HNO3 to pH<2 TN
Sodium SM 3111B Plastic 6 months HNO3 to pH<2 LA
Strontium 200.7 Plastic 6 months HNO3 to pH<2 TN
Thallium 200.7 Plastic 6 months HNO3 to pH<2 TN
Tin 200.7 Plastic 6 months HNO3 to pH<2 TN
Titanium 200.7 Plastic 6 months HNO3 to pH<2 TN
Vanadium 200.7 Plastic 6 months HNO3 to pH<2 TN
Zinc 200.7 Plastic 6 months HNO3 to pH<2 TN

Metals - ICP-MS

Tests Method Container Type Hold Time Preservation Locations Available
Aluminum 200.8 Plastic 6 months HNO3 to pH<2 TN
Antimony 200.8 Plastic 6 months HNO3 to pH<2 TN
Arsenic 200.8 Plastic 6 months HNO3 to pH<2 TN
Barium 200.8 Plastic 6 months HNO3 to pH<2 TN
Beryllium 200.8 Plastic 6 months HNO3 to pH<2 TN
Boron 200.8 Plastic 6 months HNO3 to pH<2 TN
Cadmium 200.8 Plastic 6 months HNO3 to pH<2 TN
Calcium 200.8 Plastic 6 months HNO3 to pH<2 TN
Chromium Total 200.8 Plastic 6 months HNO3 to pH<2 TN
Cobalt 200.8 Plastic 6 months HNO3 to pH<2 TN
Copper 200.8 Plastic 6 months HNO3 to pH<2 TN
Iron 200.8 Plastic 6 months HNO3 to pH<2 TN
Lead 200.8 Plastic 6 months HNO3 to pH<2 TN
Magnesium 200.8 Plastic 6 months HNO3 to pH<2 TN
Manganese 200.8 Plastic 6 months HNO3 to pH<2 TN
Molybdenum 200.8 Plastic 6 months HNO3 to pH<2 TN
Nickel 200.8 Plastic 6 months HNO3 to pH<2 TN
Phosphorous 200.8 Plastic 6 months HNO3 to pH<2 TN
Potassium 200.8 Plastic 6 months HNO3 to pH<2 TN
Selenium 200.8 Plastic 6 months HNO3 to pH<2 TN
Silver 200.8 Plastic 6 months HNO3 to pH<2 TN
Sodium 200.8 Plastic 6 months HNO3 to pH<2 TN
Strontium 200.8 Plastic 6 months HNO3 to pH<2 TN
Thallium 200.8 Plastic 6 months HNO3 to pH<2 TN
Tin 200.8 Plastic 6 months HNO3 to pH<2 TN
Titanium 200.8 Plastic 6 months HNO3 to pH<2 TN
Vanadium 200.8 Plastic 6 months HNO3 to pH<2 TN
Zinc 200.8 Plastic 6 months HNO3 to pH<2 TN

13 Priority Pollutant Metals
Sb, As, Be, Cd, Cr, Cu, Pb, Hg, Ni, Se Ag, Tl, Zn

7/40 days - 7 days to extraction, 40 days to anlaysis from extraction

Methods are subject to change.

Container Type/Holding Time/ Preservation is for information use only. Refer to 40 CFR par 136 for the most current requirements.